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The effect of therapeutical ultrasound and metalic nanoparticles on carcinoma cells
Kalužová, Adéla ; Rozman, Jiří (referee) ; Bernard, Vladan (advisor)
This thesis focuses on the research of an effect of ultrasound and nanoparticles on cancer cells. In the literature search there the applications of ultrasound field in the interaction with aplication of metallic nanoparticles to viability of cancer cells are described, on the basis of in vitro experiments‘ performance. The purpose of metallic nanoparticles‘ application should be a possible reinforcing of its antitumor effect thanks to the presence of ultrasound field. In next part of the literature search there are included the technical performance of devices, their indications and contraindications, parameters of ultrasound fields and application of metallic nanoparticles. The metallic nanoparticles have been becoming a part of the human’s life more and more, that is why it’s necessary to deal with these miniature particles at a higher level. The aim of this thesis is to suggest the ways of resolution based on the principle of sounding of cells with the ultrasound field of suitable parameters and in combination with the application of metallic nanoparticles with a successive performance of the cell viability tests. The viability was determinated by the MTT assay test. Another part of this work is the measurement of parameters of this zone by means of a submersible needle hydrophone, for a better understanding of the ultrasonic zone. In the final part of this work there is necessary the statistical evaluation of the measured data of the experiments carried out and the discussion of the results obtained.
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Ultrasonic field of level sensors measurement
Hlavatý, Radek ; Fialka, Jiří (referee) ; Beneš, Petr (advisor)
The paper deals with the description of the ultrasonic field around the piezoelectric sensors. The theoretical part serves as an overview for the understanding the ultrasound problematics. More closely studied is the phenomenon of ultrasound wave attenuation. At the end of the theoretical part, three different methods of field measuring were selected and described. The practical part of this work is a more detailed description of the whole measuring system available in the Continental laboratory, that has been put into operation. The system uses a reflection method to measure the ultrasonic field. Automated way of data processing in the form of a script in Python and a macro in Excel was designed. Uncertainties were determined by the test measurements, so was the repeatability of the measured results, that correspond to the physical theory.
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Ultrasonic field of level sensors measurement
Hlavatý, Radek ; Fialka, Jiří (referee) ; Beneš, Petr (advisor)
The paper deals with the description of the ultrasonic field around the piezoelectric sensors. The theoretical part serves as an overview for the understanding the ultrasound problematics. More closely studied is the phenomenon of ultrasound wave attenuation. At the end of the theoretical part, three different methods of field measuring were selected and described. The practical part of this work is a more detailed description of the whole measuring system available in the Continental laboratory, that has been put into operation. The system uses a reflection method to measure the ultrasonic field. Automated way of data processing in the form of a script in Python and a macro in Excel was designed. Uncertainties were determined by the test measurements, so was the repeatability of the measured results, that correspond to the physical theory.
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The effect of therapeutical ultrasound and metalic nanoparticles on carcinoma cells
Kalužová, Adéla ; Rozman, Jiří (referee) ; Bernard, Vladan (advisor)
This thesis focuses on the research of an effect of ultrasound and nanoparticles on cancer cells. In the literature search there the applications of ultrasound field in the interaction with aplication of metallic nanoparticles to viability of cancer cells are described, on the basis of in vitro experiments‘ performance. The purpose of metallic nanoparticles‘ application should be a possible reinforcing of its antitumor effect thanks to the presence of ultrasound field. In next part of the literature search there are included the technical performance of devices, their indications and contraindications, parameters of ultrasound fields and application of metallic nanoparticles. The metallic nanoparticles have been becoming a part of the human’s life more and more, that is why it’s necessary to deal with these miniature particles at a higher level. The aim of this thesis is to suggest the ways of resolution based on the principle of sounding of cells with the ultrasound field of suitable parameters and in combination with the application of metallic nanoparticles with a successive performance of the cell viability tests. The viability was determinated by the MTT assay test. Another part of this work is the measurement of parameters of this zone by means of a submersible needle hydrophone, for a better understanding of the ultrasonic zone. In the final part of this work there is necessary the statistical evaluation of the measured data of the experiments carried out and the discussion of the results obtained.
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